NMFProfiler: a multi-omics integration method for samples stratified in groups

Aurélie Mercadié, Éléonore Gravier, Gwendal Josse, Isabelle Fournier, Cécile Viodé, Nathalie Vialaneix, Céline Brouard 

bioinformatics

https://doi.org/10.1093/bioinformatics/btaf066

Abstract

Motivation: The development of high-throughput sequencing enabled the massive production of “omics” data for various applications in biology. By analyzing simultaneously paired datasets collected on the same samples, integrative statistical approaches allow researchers to get a global picture of such systems and to highlight existing relationships between various molecular types and levels. Here, we introduce NMFProfiler, an integrative supervised NMF that accounts for the stratification of samples into groups of biological interest.

Results: NMFProfiler was shown to successfully extract signatures characterizing groups with performances comparable to or better than state-of-the-art approaches. In particular, NMFProfiler was used in a clinical study on atopic dermatitis (AD) and to analyze a multi-omic cancer dataset. In the first case, it successfully identified signatures combining known AD protein biomarkers and novel transcriptomic biomarkers. In addition, it was also able to extract signatures significantly associated to cancer survival.

Availability and implementation: NMFProfiler is released as a Python package, NMFProfiler (v0.3.0), available on PyPI.