Maheul PLOTON

  • Engineers

Dr. Maheul PLOTON is a statutory engineer at INSERM. Since 2022, he has been the VVEDIT platform scientific and technical manager of the PRISM laboratory (INSERM U1192) directed by Prof. Michel SALZET.
His career began in 2004 when he joined unit U545 directed by Prof. Jean-Charles FRUCHART, he then continued in unit U1011 created in continuity by Prof. Bart STAELS. Within these units, Maheul developed his skills in the field of biochemistry, cell biology, bioinformatics and molecular biology. In 2008, he became the manager of a technical platform dedicated to the viral vectors production and use, taking charge of the technical and regulatory aspects but also the new tools development. This activity allows him to acquire solid expertise in the field of transgenesis using viral vectors. He participates in 12 publications, 7 of which are directly associated with this activity.
At the same time, Maheul has successively led two research projects, in the context of understanding the type 2 diabetes progression, which will result in publications for which he is first author. These works objectives were to determine the nuclear receptors action in pancreatic failure, “The nuclear orphan receptor Nur77 is a lipotoxicity sensor regulating glucose-induced insulin secretion in pancreatic β-cells. Mol Endocrinol. 2012”, and in the glucose production regulation by the liver “The nuclear bile acid receptor FXR is a PKA and FOXA2-sensitive activator of fasting hepatic gluconeogenesis. J Hepatol. 2018”. This last study, carried out as part of a thesis, allowed Maheul to obtain his PhD at the University of Lille.
Within the PRISM laboratory, Maheul manages the VVEDIT platform “Viral Vectors for genome EDiting and Transgenesis” on a daily basis. Viral vectors are cargo elements that allow the interest gene sequences transport and stable, transient or controlled expression within cells (in vitro, ex-vivo or in vivo). Maheul ensures the defective viral vectors custom development, optimization and production (bioengineering of promoters, gene sequences and viral tropisms, modified and optimized in order to the interest cells best target). In addition to these transversal activities, his research focuses on secondary resistance mechanisms involving tumor ECM in breast cancer and on a macrophage bioengineering method development in order to target cancer cells in glioblastoma.
Maheul is also involved in training. Beyond that dedicated to the viral vectors use, in connection with the platform, which he was able to provide to all types of personnel. He supervised two Master’s interns and technically trained many doctoral students in the various skills of which he acquired expertise.
Maheul is also a trainer within the DU “Conception en expérimentation animale (Design in animal experimentation) “. Teaching within which he carries out a course whose objectives are to explain the defective viral vector concept, to inform on the different viral vectors characteristics, to highlight their interests in in vivo transgenesis and to detail the methodological and regulatory steps of their implementation.

320434050
Avenue Paul Langevin - Bâtiment SN3 - 1er étage - Porte 105 59655 Villeneuve d'Ascq Cedex

As first author:

1: Ploton M*, Mazuy C*, Gheeraert C, Dubois V, Berthier A, Dubois-Chevalier J, Maréchal X, Bantubungi K, Diemer H, Cianférani S, Strub JM, Helleboid-Chapman A, Eeckhoute J, Staels B, Lefebvre P. The nuclear bile acid receptor FXR is a PKA and FOXA2-sensitive activator of fasting hepatic gluconeogenesis. J Hepatol. 2018 Nov;69(5):1099-1109. doi: 10.1016/j.jhep.2018.06.022. Epub 2018 Jul 5. PubMed PMID: 29981427. *Equal contribution.

2: Briand O*, Helleboid-Chapman A*, Ploton M*, Hennuyer N, Carpentier R, Pattou F, Vandewalle B, Moerman E, Gmyr V, Kerr-Conte J, Eeckhoute J, Staels B, Lefebvre P. The nuclear orphan receptor Nur77 is a lipotoxicity sensor regulating glucose-induced insulin secretion in pancreatic β-cells. Mol Endocrinol. 2012 Mar; 26(3):399-413. doi: 10.1210/me.2011-1317. Epub 2012 Feb 2. PubMed PMID: 22301783; PubMed Central PMCID: PMC5417130. * Equal contribution.

Participations:

3: Bobowski-Gerard M, Boulet C, Zummo FP, Dubois-Chevalier J, Gheeraert C, Bou Saleh M, Strub JM, Farce A, Ploton M, Guille L, Vandel J, Bongiovanni A, Very N, Woitrain E, Deprince A, Lalloyer F, Bauge E, Ferri L, Ntandja-Wandji LC, Cotte AK, Grangette C, Vallez E, Cianférani S, Raverdy V, Caiazzo R, Gnemmi V, Leteurtre E, Pourcet B, Paumelle R, Ravnskjaer K, Lassailly G, Haas JT, Mathurin P, Pattou F, Dubuquoy L, Staels B, Lefebvre P, Eeckhoute J. Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis. Nat Commun. 2022 Sep 10;13(1):5324. doi: 10.1038/s41467-022-33063-9. PMID: 36088459; PMCID: PMC9464213.

4: Dubois V, Gheeraert C, Vankrunkelsven W, Dubois-Chevalier J, Dehondt H, Bobowski-Gerard M, Vinod M, Zummo FP, Güiza F, Ploton M, Dorchies E, Pineau L, Boulinguiez A, Vallez E, Woitrain E, Baugé E, Lalloyer F, Duhem C, Rabhi N, van Kesteren RE, Chiang CM, Lancel S, Duez H, Annicotte JS, Paumelle R, Vanhorebeek I, Van den Berghe G, Staels B, Lefebvre P, Eeckhoute J. Endoplasmic reticulum stress actively suppresses hepatic molecular identity in damaged liver. Mol Syst Biol. 2020 May;16(5):e9156. doi: 10.15252/msb.20199156. PMID: 32407006; PMCID: PMC7224309.

5: Berthier A, Vinod M, Porez G, Steenackers A, Alexandre J, Yamakawa N, Gheeraert C, Ploton M, Maréchal X, Dubois-Chevalier J, Hovasse A, Schaeffer-Reiss C, Cianférani S, Rolando C, Bray F, Duez H, Eeckhoute J, Lefebvre T, Staels B, Lefebvre P. Combinatorial regulation of hepatic cytoplasmic signaling and nuclear transcriptional events by the OGT/REV-ERB complex. Proc Natl Acad Sci U S A. 2018 Nov 20;115(47): E11033-E11042. doi: 10.1073/pnas.1805397115. Epub 2018 Nov 5. PubMed PMID: 30397120; PubMed Central PMCID: PMC6255172.

6: Firmin FF, Oger F, Gheeraert C, Dubois-Chevalier J, Vercoutter-Edouart AS, Alzaid F, Mazuy C, Dehondt H, Alexandre J, Derudas B, Dhalluin Q, Ploton M, Berthier A, Woitrain E, Lefebvre T, Venteclef N, Pattou F, Staels B, Eeckhoute J, Lefebvre P. The RBM14/CoAA interacting, long intergenic non-coding RNA Paral1 regulates adipogenesis and coactivates the nuclear receptor PPAR. Sci Rep. 2017 Oct 26;7(1):14087. doi: 10.1038/s41598-017 14570-y. PubMed PMID: 29075020; PubMed Central PMCID: PMC5658386.

7: Le Guével R, Oger F, Martinez-Jimenez CP, Bizot M, Gheeraert C, Firmin F, Ploton M, Kretova M, Palierne G, Staels B, Barath P, Talianidis I, Lefebvre P, Eeckhoute J, Salbert G. Inactivation of the Nuclear Orphan Receptor COUP-TFII by Small Chemicals. ACS Chem Biol. 2017 Mar 17;12(3):654-663. doi: 10.1021/acschembio.6b00593. Epub 2017 Jan 13. PubMed PMID: 28059499.

8: Lien F, Berthier A, Bouchaert E, Gheeraert C, Alexandre J, Porez G, Prawitt J, Dehondt H, Ploton M, Colin S, Lucas A, Patrice A, Pattou F, Diemer H, Van Dorsselaer A, Rachez C, Kamilic J, Groen AK, Staels B, Lefebvre P. Metformin interferes with bile acid homeostasis through AMPK-FXR crosstalk. J Clin Invest. 2014 Mar;124(3):1037-51. doi: 10.1172/JCI68815. Epub 2014 Feb 17. PubMed PMID: 24531544; PubMed Central PMCID: PMC3938262.

9: Mazuy C, Ploton M, Eeckhoute J, Berrabah W, Staels B, Lefebvre P, Helleboid-Chapman A. Palmitate increases Nur77 expression by modulating ZBP89 and Sp1 binding to the Nur77 proximal promoter in pancreatic β-cells. FEBS Lett. 2013 Oct 25. pii: S0014-5793(13)00781-3. doi: 10.1016/j.febslet.2013.10.024. [Epub ahead of print] PubMed PMID: 24512852.

10: Berrabah W, Aumercier P, Gheeraert C, Dehondt H, Bouchaert E, Alexandre J, Ploton M, Mazuy C, Caron S, Tailleux A, Eeckhoute J, Lefebvre T, Staels B, Lefebvre P. Glucose sensing O-GlcNAcylation pathway regulates the nuclear bile acid receptor farnesoid X receptor (FXR). Hepatology. 2014 May;59(5):2022-33. doi: 10.1002/hep.26710. Epub 2014 Mar 24. PubMed PMID: 24037988.

11: Flajollet S, Rachez C, Ploton M, Schulz C, Gallais R, Métivier R, Pawlak M, Leray A, Issulahi AA, Héliot L, Staels B, Salbert G, Lefebvre P. The elongation complex components BRD4 and MLLT3/AF9 are transcriptional coactivators of nuclear retinoid receptors. PLoS One. 2013 Jun 10;8(6):e64880. doi: 10.1371/journal.pone.0064880. Print 2013. PubMed PMID: 23762261; PubMed Central PMCID: PMC3677938.

12: Caron S, Huaman Samanez C, Dehondt H, Ploton M, Briand O, Lien F, Dorchies E, Dumont J, Postic C, Cariou B, Lefebvre P, Staels B. Farnesoid X receptor inhibits the transcriptional activity of carbohydrate response element binding protein in human hepatocytes. Mol Cell Biol. 2013 Jun;33(11):2202-11. doi: 10.1128/MCB.01004-12. Epub 2013 Mar 25. PubMed PMID: 23530060; PubMed Central PMCID: PMC3648076.

13: Delhon C, Bernigaud N, Berger J.-F, Salvador P.-G, Thiébault S & Ploton M (2013). Evolution and management of humid landscapes in northern Dauphiné (Rhone valley, France): Contribution of charcoal and wood studies. The Holocene, 23(10), 1447–1465.