Antonella Raffo Romero

  • PostDoc

is a postdoctoral researcher at the Université de Lille, Inserm, CHU Lille, in the PRISM Laboratory – U1192 Inserm (Protéomique Réponse Inflammatoire Spectrométrie de Masse) in Lille, France. She specialises in 3D cell culture, in particular organoid models and bioprinting development, with a focus on breast cancer. Her research aims to create innovative models that closely mimic in vivo conditions to study the complex interactions between cancer cells and immune cells, in particular macrophages, in cancer progression and response to therapy. These models are developed within the 3D CellOmics platform, part of the OrganOmics platform, where she is the technical coordinator of the 3D CellOmics section.
Dr Raffo-Romero has published 20 scientific articles, five as first author and one as corresponding author. She is involved in several bioprinting development projects and has obtained three major funding sources: one national funding (2023 FC3R Replacement) and two regional funding sources (2023 AAP CPER TecSanté and 2021-2022 Cancéropôle Nord-Ouest). She has won two awards for the best oral presentation (in 2022 and 2017) and three grants to attend congresses (from 2022 to 2024).
In addition to her research, Dr Raffo-Romero contributes to education by teaching 3D cell culture at the École Centrale and mentoring students. She has supervised 10 students at different levels (Master, Bachelor, DUT). She is also an active member of networks such as CPER TecSanté and GDR organoids, where she is part of the organising committee for the GDR organoid webinars. Besides research and teaching, Dr Raffo-Romero has been active in organising scientific events such as the SMAP 2024 conference.

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1. Protocol for generating a co-culture of macrophages with breast cancer tumoroids. Raffo-Romero A, Ziane-Chaouche L, Hajjaji N, Salzet M, Duhamel M.
2. Inhibition of furin in CAR macrophages directs them toward a proinflammatory phenotype and enhances their antitumor activities. Lydia Ziane-Chaouche, Antonella Raffo-Romero, Nawale Hajjaji, Firas Kobeissy, Donna Pinheiro, Soulaimane Aboulouard, Adeline Cozzani, Suman Mitra, Isabelle Fournier, Dasa Cizkova, Michel Salzet & Marie Duhamel
3. A co-culture system of macrophages with breast cancer tumoroids to study cell interactions and therapeutic responses. Raffo-Romero A, Ziane-Chaouche L, Salomé-Desnoulez S, Hajjaji N, Fournier I, Salzet M, Duhamel M.
4. Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome. Alice Capuz, Sylvain Osien, Tristan Cardon, Mélodie Anne Karnoub, Soulaimane Aboulouard, Antonella Raffo-Romero, Marie Duhamel, Dasa Cizkova, Marco Trerotola, David Devos, Firas Kobeissy, Fabien Vanden Abeele, Amélie Bonnefond, Isabelle Fournier, Franck Rodet, Michel Salzet.
5. Astrocytes express aberrant immunoglobulins as putative gatekeeper of astrocytes to neuronal progenitor conversion. Alice Capuz, Sylvain Osien, Mélodie Anne Karnoub, Soulaimane Aboulouard, Estelle Laurent, Etienne Coyaud, Antonella Raffo-Romero, Marie Duhamel, Amélie Bonnefond, Mehdi Derhourhi, Marco Trerotola, Ikram El Yazidi-Belkoura, David Devos, Monika Zilkova, Firas Kobeissy, Fabien Vanden Abeele, Isabelle Fournier, Dasa Cizkova, Franck Rodet, Michel Salzet.
6. Establishment and characterization of canine mammary tumoroids for translational research. Antonella Raffo-Romero, Soulaimane Aboulouard, Emmanuel Bouchaert, Agata Rybicka, Dominique Tierny, Nawale Hajjaji, Isabelle Fournier, Michel Salzet, Marie Duhamel.
7. On-tissue spatially resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape. Malaker SA, Quanico J, Raffo-Romero A, Kobeissy F, Aboulouard S, Tierny D, Bertozzi CR, Fournier I, Salzet M.
8. Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment. Lemaire Q, Duhamel M, Raffo-Romero A, Salzet M, Lefebvre C.
9. Location of neonatal microglia drives small extracellular vesicles content and biological functions in vitro. Murgoci AN, Duhamel M, Raffo-Romero A, Mallah K, Aboulouard S, Lefebvre C, Kobeissy F, Fournier I, Zilkova M, Maderova D, Cizek M, Cizkova D, Salzet M.
10. Cross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program. Geirsdottir L, David E, Keren-Shaul H, Weiner A, Bohlen SC, Neuber J, Balic A, Giladi A, Sheban F, Dutertre CA, Pfeifle C, Peri F, Raffo-Romero A, Vizioli J, Matiasek K, Scheiwe C, Meckel S, Mätz-Rensing K, van der Meer F, Thormodsson FR, Stadelmann C, Zilkha N, Kimchi T, Ginhoux F, Ulitsky I, Erny D, Amit I, Prinz M.
11. Isolation of microglia-derived extracellular vesicles: towards miRNA signatures and neuroprotection. Lemaire Q, Raffo-Romero A, Arab T, Van Camp C, Drago F, Forte S, Gimeno JP, Begard S, Colin M, Vizioli J, Sautière PE, Salzet M, Lefebvre C.
12. PC1/3 KD Macrophages Exhibit Resistance to the Inhibitory Effect of IL-10 and a Higher TLR4 Activation Rate, Leading to an Anti-Tumoral Phenotype. Rodet F, Capuz A, Ozcan BA, Le Beillan R, Raffo-Romero A, Kobeissy F, Duhamel M, Salzet M.
13. Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Lipids in Experimental Model of Traumatic Brain Injury Detecting Acylcarnitines as Injury Related Markers. Mallah K, Quanico J, Raffo-Romero A, Cardon T, Aboulouard S, Devos D, Kobeissy F, Zibara K, Salzet M, Fournier I.
14. Mapping Spatiotemporal Microproteomics Landscape in Experimental Model of Traumatic Brain Injury Unveils a link to Parkinson’s Disease. Mallah K, Quanico J, Raffo-Romero A, Cardon T, Aboulouard S, Devos D, Kobeissy F, Zibara K, Salzet M, Fournier I.
15. Proteomic characterisation of leech microglia extracellular vesicles (EVs): comparison between differential ultracentrifugation and Optiprep™ density gradient isolation. Arab T, Raffo-Romero A, Van Camp C, Lemaire Q, Le Marrec-Croq F, Drago F, Aboulouard S, Slomianny C, Lacoste AS, Guigon I, Touzet H, Salzet M, Fournier I, Lefebvre C, Vizioli J, Sautière PE.
16. ALK4/5-dependent TGF-β signaling contributes to the crosstalk between neurons and microglia following axonal lesion. Raffo-Romero A, Arab T, Van Camp C, Lemaire Q, Wisztorski M, Franck J, Aboulouard S, Le Marrec-Croq F, Sautiere PE, Vizioli J, Salzet M, Lefebvre C.
17. Medicinal Leech CNS as a Model for Exosome Studies in the Crosstalk between Microglia and Neurons. Raffo-Romero A, Arab T, Al-Amri IS, Le Marrec-Croq F, Van Camp C, Lemaire Q, Salzet M, Vizioli J, Sautiere PE, Lefebvre C.
18. 3D MALDI mass spectrometry imaging reveals specific localization of long-chain acylcarnitines within a 10-day time window of spinal cord injury. Quanico J, Hauberg-Lotte L, Devaux S, Laouby Z, Meriaux C, Raffo-Romero A, Rose M, Westerheide L, Vehmeyer J, Rodet F, Maass P, Cizkova D, Zilka N, Cubinkova V, Fournier I, Salzet M.
19. Deciphering molecular consequences of the proprotein convertase 1/3 inhibition in macrophages for application in anti-tumour immunotherapy. Rodet F, Capuz A, Hara T, van Meel R, Duhamel M, Rose M, Raffo-Romero A, Fournier I, Salzet M.
20. Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions. Delcourt V, Franck J, Quanico J, Gimeno JP, Wisztorski M, Raffo-Romero A, Kobeissy F, Roucou X, Salzet M, Fournier I.