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Christelle Van Camp

Technician at University Lille 1

University Lille 1
Bât SN3, 1er étage, porte 103

Tel : +33 (0)3 20 43 68 39
Fax : +33 (0)3 20 43 40 56

Email : Christelle.Van-Camp@univ-lille1.fr

CURRENT SITUATION
Technician in the Microglial Activation Axis of the laboratory

CURSUS
- 1992-1993 : First year of PhD Thesis (Poitiers University of Sciences), Biomembranes speciality, option : Cellular and Molecular Biology, Physiology. (Poitou-Charentes region grant).
- 1992 : Master of Sciences « Biomembranes » (MSC, Poitiers University of Sciences), option II : Membranes Physiology and Pharmacology, Cellular interactions. (MSC grant of French government).

FUNCTIONS
Missions :
- Responsible of managment of material consumable of the laboratory, and of the products of in situ Hybridization sector
- Establish business connections, quotation requests and the orders of these 2 sectors
- Assure the centralization of experimental processes
- Responsible of the biological material
- Precise dissection of the leech nervous system (Hirudo Medicinalis)
- Preparation of cellular and molecular samples
- Preparation of tissues, and paraffin and frozen sections
- Preparation of samples for the study in flow cytometry
- Analysis of pictures in photonic microscopy
- welcome and supervisor of the new students for the discovery and the driving of experimentations corresponding to my technical missions, and transmission of my skills during the formation of student researchers
Technical skills :
- Sample protein extraction, protein determination by Bradford method
- Western Blotting (SDS PAGE) and Dot Blotting
- Trypsic digestion and Zip tip
- Synthesis of labelled RNA probes and in situ hybridization
- Total mRNA extraction, retrotranscription, QPCR
- Immunohistochemistry on cultured cells, on paraffin, frozen, semi thin sections and in toto tissues
- Immunoprecipitation and Co-immunoprecipitation
- chemotaxis assay
- Primary culture of nerve cells (neurons and microglial cells)
- Organotypic cultures of Leech nerve cord (Hirudo Medicinalis)
- Tissues coloration
Professional formations :
- Nov 2012 : Formation to Flow Cytometry level 1.
- Sept 2011 : Formation to Animal Experimentation level 2.

PUBLICATIONS
Voisin P, Van Camp G, Pontoire C (Van Camp C), Collin JP : Prostaglandins stimulate serotonin acetylation in chick pineal cells : involvement of cyclic AMP-dependent and calcium /calmodulin-dependent mechanisms. J Neurochem. 1993 Feb, 60(2) : 666-70.
Pontoire C (Van Camp C), Bernard M, Silvain C, Collin JP, Voisin P : Characterization of melatonin binding sites in chicken and human intestines. Eur J Pharmacol. 1993 Oct 15, 247 (2) : 111-8.
Tasiemski A, Schikorski D, Le Marrec-Croq F, Pontoire-Van Camp C, Boidin-Wichlacz C, Sautière PE. 2006. Hedistin: A novel antimicrobial peptide containing bromotryptophan constitutively expressed in the NK cells-like of the marine annelid, Nereis diversicolor. Dev.Comp.Immunol; 31(8):749-62.
Lemaire R., Stauber J., Wisztorski M., Van Camp C., Desmons A., Deschamps M., Proess G., Rudlof I., Woods AS., Day R., Salzet M., Fournier I. 2007. Tag-mass : specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag. J. proteome Res. ; 6(6) : 2057-67.
Tahtouh M, Croq F, Vizioli J, Van Camp C, Salzet M, Daha M, Lefebvre C, Pestel J: A C1q domain containing factor evidenced in the leech nerve cord expressed a chemotactic activity on microglial cells. Mol Immunol 2008, 45:4141-4141.
Tahtouh M, Croq F, Vizioli J, Sautiere PE, Van Camp C, Salzet M, Daha MR, Pestel J, Lefebvre C: Evidence for a novel chemotactic C1q domain-containing factor in the leech nerve cord. Mol Immunol 2009, 46:523-531.
J Vizioli, F Croq, A Garçon-Bocquet, M Tahtouh, P-E Sautière, C Van Camp, M Salzet, J Pestel and C Lefebvre. Implication of an IL-16 homologous in microglial cells recruitment after CNS injury in the medicinal leech Hirudo medicinalis. XIth Meeting of the Italian Society of Development and Comparative Immunity (ISDCI). February 2010, (Modena, Italy). ISJ, 7: 107-118, 2010
Croq F, Vizioli J, Tuzova M, Tahtouh M, Sautiere PE, Van Camp C, Salzet M, Cruikshank WW, Pestel J, Lefebvre C: A homologous form of human interleukin 16 is implicated in microglia recruitment following nervous system injury in leech Hirudo medicinalis. Glia 2010, 58:1649-1662.
J Vizioli, S Giacomelli, C. Van Camp, MR Coscia, U Oreste: Immunoglobulins at the Blood-Brain Barrier in Trematomus bernacchii. XIIth Scientific meeting of the Italian Association of Developmental and Comparative Immunobiology (IADCI) 16-18 February 2011, Padua, Italy.ISJ, 8: 33-47, 2011.
Croq F, Garçon-Bocquet A, Vizioli J, Van Camp C, Sautiere PE, Salzet M, Lefebvre C: Microglia Activation: Studies on Leeches to Dissect out the Basic Mechanisms of Neuroinflammation. Glia 2011, 59:S135-S135.
Vizioli J, Accorsi A, Croq F, Bocquet-Garçon A, Van Camp C, Ziental N, Salzet M, Lefebvre C, Sautiere PE: Neuroinflammation and Microglia Activation Studies: a Novel Strategy Using an Invertebrate Model, the Medicinal Leech. Glia 2011, 59:S153-S153.
F. Drago *1, T. Schorn *2, A. Accorsi 3, P.E. Sautière1, F. Croq 1, C. Lefebvre 1, C. Van Camp 1, A. Grimaldi 2, J. Vizioli 1. Identification and modulation of an Allograft Inflammatory Factor-1 (AIF-1) homologous in the medicinal leech, Hirudo medicinalis. XIIIth Scientific meeting of the Italian Association of Developmental and Comparative Immunobiology (IADCI) 22-24 February 2012, S. Benedetto del Tronto, Italy. ISJ, 9: 35-47, 2012.
Tahtouh M, Garçon-Bocquet A, Croq F, Vizioli J, Sautiere PE, Van Camp C, Salzet M, Nagnan-Le Meillour P, Pestel J, Lefebvre C: Interaction of HmC1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis. J Neuroinflammation 2012, 9:37.

CONGRESS PARTICIPATION
Garçon-Bocquet A, Croq F, Vizioli J, Tahtouh M, Van Camp C, Nagnan-Le Meillour P, Salzet M, Sautière PE, Lefebvre C. Microglia recruitment during the CNS repair in the medicinal leech, Hirudo medicinalis. 12th Symposium on Invertebrate Neurobiology. Août 2011, Tihany, Hungary.